Microbial reference strains are essential tools in quality control, research, and diagnostic laboratories. However, their proper handling requires strict adherence to biosafety protocols to ensure both user safety and culture integrity.
Understanding Reference Strains
Reference strains are well-characterized microorganisms with known properties. Organizations like ATCC (American Type Culture Collection) maintain these strains under controlled conditions to ensure genetic stability and phenotypic consistency.
Key Safety Considerations
- Biosafety Level Assessment: Always check the biosafety level (BSL) of the organism before handling. Most quality control strains are BSL-1 or BSL-2.
- Personal Protective Equipment: Use appropriate PPE including lab coats, gloves, and eye protection.
- Aseptic Technique: Work in a laminar flow hood or biosafety cabinet when handling cultures.
- Waste Disposal: Autoclave all culture materials before disposal.
Storage and Revival
Most reference strains are shipped in freeze-dried or frozen formats. Follow manufacturer guidelines for:
- Storage temperature requirements (-80°C or liquid nitrogen)
- Revival procedures and appropriate media
- Subculturing limits to maintain strain integrity
Documentation
Maintain detailed records of:
- Lot numbers and certificates of analysis
- Date of receipt and revival
- Passage number and storage location
- Any deviations or observations
By following these guidelines, laboratories can ensure safe handling while maintaining the quality and reliability of their reference strain cultures.